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Example 6: Calibration

General introduction to the calibration

We offer two calibration methods: an internal and an external calibration. Both can handle peak data as well as raw data. If you want to calibrate raw data, a peak picking step is necessary, the important parameters can be set via the ini-file. If you have already picked data, don't forget the '-peak_data' flag.

The external calibration is used to convert flight times into m/z- values with the help of external calibrant spectra containing e.g. a polymer like polylysine. For the calibrant spectra, the calibration constants the machine uses need to be known as well as the expected masses. Then a quadratic function is fitted to convert the flight times into m/z-values.

The internal calibration uses reference masses in the spectra to correct the m/z-values using a linear function.


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